Observing live actin in the human trabecular meshwork
نویسندگان
چکیده
منابع مشابه
Hyaluronan in the human trabecular meshwork.
PURPOSE Hyaluronan (HA) is a high-molecular weight glycosaminoglycan that can affect water and solute fluxes in the extracellular matrix. The distribution of HA in the human trabecular meshwork of nonglaucomatous eyes was examined to help understand the potential role of HA in the regulation of aqueous outflow resistance. METHODS Histolocalization of HA was established in situ in the trabecul...
متن کاملErythrocyte phagocytosis in the human trabecular meshwork.
The mechanisms of erythrocyte disposal by the trabecular meshwork in the human eye have not been extensively studied by electron microscopy. At the present time there is one short report on the ultrastructural changes induced by post-traumatic recurrent hyphaemas in two elderly patients. In both patients, Yamashita and Rosen ( I965) observed infiltration of the endothelial meshwork by phagocyti...
متن کاملsCD44 internalization in human trabecular meshwork cells.
PURPOSE To determine whether soluble CD44 (sCD44), a likely biomarker of primary open-angle glaucoma (POAG), is internalized in cultured human trabecular meshwork (TM) cells and trafficked to mitochondria. METHODS In vitro, 32-kD sCD44 was isolated from human sera, biotinylated, and dephosphorylated. TM cells were incubated for 1 hour at 4°C with biotinylated albumin (b-albumin), biotin-label...
متن کاملMechanical stretch alters the actin cytoskeletal network and signal transduction in human trabecular meshwork cells.
PURPOSE Human trabecular meshwork (HTM) cells were mechanically stretched in vitro as a potential model for the distension of this tissue that can occur in vivo in response to increased pressure gradients. Cell morphology and certain components of the signal transduction pathways, including the mitogen-activated protein kinase (MAPK) and c-Jun N-terminal protein kinase (JNK) pathways, were eval...
متن کاملIntegrins Cooperate to Induce Syndecan-4-Containing Cross-linked Actin Networks in Human Trabecular Meshwork Cells
METHODS. Immunofluorescence microscopy was used to identify molecular components of CLANs and quantitate CLAN formation in HTM cells plated on coverslips coated with various extracellular matrix (ECM) proteins (fibronectin, types I and IV collagen, and vitronectin), vascular cell adhesion molecule (VCAM)-1, or activating antibodies against 1, 3, or 2 1 integrins. These integrin antibodies were ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical & Experimental Ophthalmology
سال: 2013
ISSN: 1442-6404
DOI: 10.1111/ceo.12255